Antimicrobial activity of the combination (Nano-Bio) of Artemisia absinthium with copper nanoparticles

Ozgur Celebi^a, Kagan Tolga Cinisli^a, Demet Celebi^b

Atatürk University, Faculty of Medicine, Department of Medical Microbiology, Erzurum, 25240, Turkey.

Abstract

Nanoparticles show the lowest level of toxicity in the life cycle and ecosystem. Therefore, using these substances to combat pathogens may be an appropriate choice. Artemisia absinthium is traditionally used as an anthelmintic, antiseptic, antispasmodic, and worldwide for bacillus dysentery. The oil composition was analyzed by gas chromatography: mass spectrometry. Then, a nano-bio formulation was formed by inoculating a copper nanoparticle (50, 100 μg / ml) into the plant oil extract. Microorganisms were provided by Atatürk University Research Hospital. Examples were Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus epidermidis, Acinetobacter baumannii, Staphylococcus aureus, Enterobacter aerugenes, Candida albicans, and E.coli. Minimum inhibitory concentration values were determined for Pseudomonas aeruginosa (MIC = 500 μg / ml), Klebsiella pneumoniae (MIC = 500 μg / ml), Proteus mirabilis (MIC = 500 μg / ml). Staphylococcus epidermidis (MIC < 1.95 μg / ml), Acinetobacter baumannii (MIC = 500 μg / ml), Staphylococcus aureus (MIC < 125 μg / ml), Enterobacter aerugenes (MIC = 500 μg / ml), E.coli (MIC = 500 μg / ml), Candida albicans (MIC = 500 μg / ml), respectively. Anti-microbial activity of camphor extract of essential oil was tested by disk diffusion method. Plant extracts should be considered when used in part of the substitution process. A stock suspension was prepared by resuspending the nanoparticles in double distilled water to obtain a final concentration. And artemisia mixed CuO nanoparticle was determined by applying standard bacteriological methods with agar dilution method against 9 microorganisms isolated. Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus epidermidis, Acinetobacter baumannii, Staphylococcus aureus, Enterobacter aerugenes, Candida albicans, and E.coli. Values (μg / ml) were 50, 100 copper. Their use as antimicrobial compounds to combat a large number of resistant pathogens is promising for alternative therapy.

Keywords: Copper nanoparticles, Nano-bio, Artemisia absinthium, Antimicrobial.